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Inventi Impact: Medicine

Articles

  • Inventi:hhm/20001/16
    An Optimized Injectable Hydrogel Scaffold Supports Human Dental Pulp Stem Cell Viability and\nSpreading
    01-Jan-1970 Research 2016 : July - September
    T D Jones, A Kefi, S Sun, M Cho, S B Alapati

    Introduction. HyStem-C is a commercially available injectable hydrogel composed of polyethylene glycol diacrylate (PEGDA),\nhyaluronan (HA), and gelatin (Gn). These components can be mechanically tuned to enhance cell viability and spreading. Methods.\nThe concentration of PEGDA with an added disulfide bond (PEGSSDA) was varied from 0.5 to 8.0% (w/v) to determine the\noptimal concentration for injectable clinical application.We evaluated the cell viability of human dental pulp stem cells (hDPSCs)\nembedded in 2% (w/v) PEGSSDA-HA-Gn hydrogels. Volume ratios of HA:Gn from 100 : 0 to 25 : 75 were varied to encourage\nhDPSC spreading. Fibronectin (Fn) was added to our model to determine the effect of extracellular matrix protein concentration\non hDPSC behavior. Results. Our preliminary data suggests that the hydrogel gelation time decreased as the PEGSSDA cross-linker\nconcentration increased. The PEGSSDA-HA-Gn was biocompatible with hDPSCs, and increased ratios of HA:Gn enhanced cell\nviability for 14 days. Additionally, cell proliferation with added fibronectin increased significantly over time at concentrations of\n1.0 and 10.0

    How to Cite this Article
    CC Compliant Citation: T. D. Jones, A. Kefi, S. Sun, M. Cho, and S. B. Alapati, â??An Optimized Injectable Hydrogel Scaffold Supports Human\nDental Pulp Stem Cell Viability and Spreading,â? Advances in Medicine, vol. 2016, Article ID 7363579, 8 pages, 2016.\ndoi:10.1155/2016/7363579, http://creativecommons.org/licenses/by/4.0/.
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